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EVIDENCE OF ZEIN-BOUND INDOLEACETIC-ACID USING GAS CHROMATOGRAPHY-SELECTED ION MONITORING-MASS SPECTROMETRY ANALYSIS AND IMMUNOGOLD LABELING

被引：7
作者：
LEVERONE, LA
KOSSENJANS, W
JAYASIMIHULU, K
CARUSO, JL
机构：
[1] UNIV CINCINNATI,DEPT BIOL SCI,CINCINNATI,OH 45221
[2] UNIV CINCINNATI,DEPT PATHOL & LAB MED,CINCINNATI,OH 45267
[3] UNIV CINCINNATI,DEPT ENVIRONM HLTH,CINCINNATI,OH 45267
来源：
PLANT PHYSIOLOGY | 1991年 / 96卷 / 04期
关键词：
D O I：
10.1104/pp.96.4.1070
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Commercial zein was base-hydrolyzed and purified extracts were subjected to gas chromatography-selected ion monitoring-mass spectrometry analysis. Indoleacetic acid (IAA) was shown to be released from this storage protein of corn (Zea mays). Isotope dilution using [C-13(6)]IAA as an internal standard revealed a conservative ratio of 1 mole IAA to 175 moles zein. Immunoelectron micrographs of isolated protein bodies also showed IAA or an IAA-like molecule associated with zein and deposited within these organelles.
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收藏
页码：1070 / 1075
页数：6
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