DETERMINATION OF TRANSFER RNA(PHE) RECOGNITION NUCLEOTIDES FOR PHENYLALANYL-TRANSFER RNA-SYNTHETASE FROM THERMUS-THERMOPHILUS

被引：19

作者：

MOOR, N ^{[1
]}

NAZARENKO, I ^{[1
]}

ANKILOVA, V ^{[1
]}

KHODYREVA, S ^{[1
]}

LAVRIK, O ^{[1
]}

机构：

[1] NOVOSIBIRSK STATE UNIV,NOVOSIBIRSK 630090,USSR

来源：

BIOCHIMIE | 1992年 / 74卷 / 04期

关键词：

PHENYLALANYL-TRANSFER RNA SYNTHETASE; TRANSFER RNA IDENTITY POINTS;

D O I：

10.1016/0300-9084(92)90112-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tRNA(Phe) recognition nucleotides for phenylalanyl-tRNA synthetase from an extreme thermophile Thermus thermophilus were investigated. Using yeast tRNA(Phe) T7 transcripts with various point mutations it was shown that four recognition points (G34, A35, A36 from anticodon and A73 from acceptor stem) are important for aminoacylation at 37-degrees-C. In the case of the 73rd discriminator base A-->U, but not A-->C, substitution suppresses aminoacylation. Position 20, which proved to be essential for all phenylalanyl-tRNA synthetases so far studied, is not involved in the recognition of tRNA(Phe) by the T thermophilus enzyme.

引用

页码：353 / 356

页数：4

共 9 条

[1] PHENYLALANYL-TRANSFER RNA-SYNTHETASE FROM THERMUS-THERMOPHILUS HB8 - PURIFICATION AND PROPERTIES OF THE CRYSTALLIZING ENZYME [J].