CONTRASTING EFFECTS OF PACAP AND CARBACHOL ON [CA-2+]I AND INOSITOL PHOSPHATES IN HUMAN NEUROBLASTOMA NB-OK-1 CELLS

被引：26

作者：

DELPORTE, C

VANPRAET, A

HERCHUELZ, A

WINAND, J

CHRISTOPHE, J

机构：

[1] UNIV LIBRE BRUXELLES,SCH MED,DEPT BIOCHEM & NUTR,BLDG GE,CP 611,ROUTE LENNIK 808,B-1070 BRUSSELS,BELGIUM

[2] UNIV LIBRE BRUXELLES,SCH MED,DEPT PHARMACOL,B-1070 BRUSSELS,BELGIUM

来源：

PEPTIDES | 1993年 / 14卷 / 06期

关键词：

PACAP RECEPTORS; MUSCARINIC RECEPTORS; CYTOSOLIC FREE CALCIUM; INOSITOL PHOSPHATES; HUMAN NEUROBLASTOMA CELLS NB-OK-1;

D O I：

10.1016/0196-9781(93)90163-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of PACAPs on [Ca2+]i were compared to those of carbachol in human neuroblastoma NB-OK-1 cells. PACAP(1-27) and PACAP(1-38) increased [Ca2+]i in a biphasic manner: a transient rise and a secondary plateau. The transient phase reflected the mobilization of [Ca2+]i pool(s) via the inositol phosphate pathway. The modest sustained plateau required extracellular Ca2+. Carbachol also increased [Ca2+]i in a biphasic manner, but it mobilized intracellular Ca2+ pool(s) with a higher efficacy than PACAPs, then greatly increased Ca2+ entry, this being accompanied by a more marked and prolonged elevation of IP3 and IP4 than with PACAPs. It is likely that cAMP-mediated phosphorylations due to PACAPs facilitated desensitization at the PACAP receptor-phospholipase C level, so that there was less Ca2+ handling through PACAP receptors than with muscarinic M1 receptors.

引用

页码：1111 / 1118

页数：8

共 37 条

[1] EXPRESSION CLONING OF A COMMON RECEPTOR FOR PARATHYROID-HORMONE AND PARATHYROID HORMONE-RELATED PEPTIDE FROM RAT OSTEOBLAST-LIKE CELLS - A SINGLE RECEPTOR STIMULATES INTRACELLULAR ACCUMULATION OF BOTH CAMP AND INOSITOL TRISPHOSPHATES AND INCREASES INTRACELLULAR FREE CALCIUM [J].