A TRANSPOSABLE EPITOPE OF HLA-B7, B40 MOLECULES

被引：50

作者：

WAYS, JP ^{[1
]}

LAWLOR, DA ^{[1
]}

WAN, AM ^{[1
]}

PARHAM, P ^{[1
]}

机构：

[1] STANFORD UNIV, DEPT CELL BIOL, STANFORD, CA 94305 USA

来源：

IMMUNOGENETICS | 1987年 / 25卷 / 05期

关键词：

D O I：

10.1007/BF00404425

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The monoclonal antibody MB40.2 defines a novel subtype of HLA-B40 that is expressed by the Sweig cell line. This molecule, called HLA-B40*, lacks an antigenic determinant that is common to HLA-B7 and the HLA-Bw60 subtype of HLA-B40. Genes encoding HLA-B40* and HLA-Bw60 have now been isolated and the amino acid sequences of these proteins compared with other HLA-B locus molecules. These results show that HLA-B40* is a unique protein which differs from HLA-Bw60 by eight amino acid substitutions. Comparison of the sequences for HLA-B40*, -Bw60, and -B7 localizes the MB40.2 epitope to a cluster of three substitutions at positions 177, 178, and 180 at the end of the .alpha.2 domain. Gene conversion or reciprocal recombination are postulated to have transferred this cluster of substitutions, and their associated epitope, during the evolution of HLA-B locus genes. The epitope may consist of an .alpha. helical segment which is exclusively found on MB40.2 positive molecules.

引用

页码：323 / 328

页数：6

共 30 条

[1]

Chou P Y, 1978, Adv Enzymol Relat Areas Mol Biol, V47, P45

[2] HLA-B LOCUS POLYMORPHISM - STUDIES WITH A SPECIFIC HYBRIDIZATION PROBE [J].