PROTEIN PROFILES OF MICROSECTIONS OF THE FETAL AND ADULT HUMAN LENS DURING DEVELOPMENT AND AGING

被引：13

作者：

BOURS, J ^{[1
]}

WEGENER, A ^{[1
]}

HOFMANN, D ^{[1
]}

FODISCH, HJ ^{[1
]}

HOCKWIN, O ^{[1
]}

机构：

[1] UNIV BONN,INST PEDIAT PATHOL,W-5300 BONN 1,GERMANY

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 1990年 / 54卷 / 01期

关键词：

Agarose/polyacrylamide isoelectric focusing; Ageing; Development; Frozen-sectioning technique; HM-; α-; pre-α; β-; and; γ-crystallins; Human lens; Water-soluble crystallins;

D O I：

10.1016/0047-6374(90)90012-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The water-soluble proteins of the human fetal lens (175- and 285-day-old) contain HM-, pre-α, α-, β- and γ-crystallins. Using the frozen-sectioning technique, it can be demonstrated that the fetal lens does not have a homogeneous distribution of crystallins, but there are gradual differences between the cortices and the nucleus. The frozen-sectioning technique shows for the adult lens significantly increasing amounts of β-crystallins of pI 4.95-5.5, especially at the posterior supra-nuclear layer, increasing amounts of HM-crystalins and decreasing amounts of β-crystallins of pI 5.80-7.05 in the nucleus. This microsectioning technique was correlated with Scheimpflug photographs of the fetal and adult lens. In the fetal lens, the anterior capsule and 2 peaks in the anterior and posterior supranuclear layers could be visualized after densitometry. In the adult lens 5 layers could be demonstrated, e.g. the anterior capsule, the anterior supranuclear layer, the nucleus, the posterior supranuclear layer and the anterior capsule. © 1990.

引用

页码：13 / 27

页数：15

共 27 条

[1]

AHREND M, 1985, OPHTHALMIC RES, V17, P197

[2] WATER-SOLUBLE AND INSOLUBLE CRYSTALLINS OF THE DEVELOPING HUMAN-FETAL LENS, ANALYZED BY AGAROSE POLYACRYLAMIDE THIN-LAYER ISOELECTRIC-FOCUSING [J].