CALLUS FORMATION AND PLANT-REGENERATION FROM PROTOPLASTS DERIVED FROM SUSPENSION-CULTURE OF RICE (ORYZA-SATIVA L CV TAIPEI 177)

被引：4

作者：

BISWAS, GCG ^{[1
]}

ZAPATA, FJ ^{[1
]}

机构：

[1] INT RICE RES INST, POB 933, MANILA, PHILIPPINES

来源：

PLANT BREEDING | 1990年 / 105卷 / 02期

关键词：

ORYZA-SATIVA L; PROTOPLASTS; CALLUS FORMATION; PLANT REGENERATION;

D O I：

10.1111/j.1439-0523.1990.tb00460.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Protoplasts were isolated from young inflorescence-derived suspension cultures of a japonica rice cultivar "Taipei 177". The isolated protoplasts which were cultured either in liquid, agar on Sea plaque agarose underwent sustained division. Maximum plating efficiency of 1.06% occurred in a medium containing macroelements of KM, microelements and vitamins of B5, 0.5% Sea plaque agarose, 1.0 mg/l of 2,4-D, and glucose as an osmotic stabilizer. Green and albino plants were regenerated from the protocalli in MS semisolid medium containing 4 mg/l BAP, 0.5 mg/l NAA and 500 mg/l casein hydrolysate (MS18-2).

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页码：95 / 100

页数：6

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