IMMOBILIZED ENZYME CARBON PASTE ELECTRODES AS AMPEROMETRIC SENSORS

被引：20

作者：

LORENZO, E ^{[1
]}

GONZALEZ, E ^{[1
]}

PARIENTE, F ^{[1
]}

HERNANDEZ, L ^{[1
]}

机构：

[1] AUTONOMOUS UNIV MADRID,DEPT ANALYT CHEM & INSTRUMENTAL ANAL,E-28049 MADRID,SPAIN

来源：

ELECTROANALYSIS | 1991年 / 3卷 / 4-5期

关键词：

D O I：

10.1002/elan.1140030412

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The construction and performance of new carbon paste enzyme electrodes as amperometric sensors is described. Xanthine oxidase and alkaline phosphatase have been adsorbed on a carbon paste electrode (CPE), and physically entrapped with a semipermeable membrane. The product of enzymatic reaction, uric acid or p-nitrophenol, was electrochemically oxidized versus an Ag/AgCl electrode at + 0.4 or + 0.8 V, respectively, yielding a steady-state current directly related to the substrate concentration. Response characteristics as well as kinetic parameters have been evaluated.

引用

页码：319 / 323

页数：5

共 24 条

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