COMPARISON OF THE GEN-PROBE PACE-2 SYSTEM, DIRECT FLUORESCENT-ANTIBODY, AND CELL-CULTURE FOR DETECTING CHLAMYDIA-TRACHOMATIS IN CERVICAL SPECIMENS

被引：35

作者：

IWEN, PC ^{[1
]}

BLAIR, TMH ^{[1
]}

WOODS, GL ^{[1
]}

机构：

[1] UNIV NEBRASKA,MED CTR,DEPT SURG EMERGENCY MED,OMAHA,NE

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 1991年 / 95卷 / 04期

关键词：

CHLAMYDIA-TRACHOMATIS; DNA PROBE; LABORATORY DIAGNOSIS; CLINICAL DIAGNOSIS;

D O I：

10.1093/ajcp/95.4.578

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A chemiluminescent-labeled DNA probe (Gen-Probe PACE(TM) 2 System) for detection of Chlamydia trachomatis in endocervical specimens was evaluated. For each specimen, tissue cell culture, direct immunofluorescent staining (DFA), and DNA probe assay were performed. Thirty of 318 (9.4%) specimens were positive for C. trachomatis. The sensitivities, specificities, and positive and negative predictive values of the DNA probe compared with cell culture were 93%, 98%, 85%, and 99%, respectively, and for DFA these same values were 81%, 99%, 83%, and 99%, respectively. The Gen-Probe PACE 2 Systems is a reliable method for the rapid detection of endocervical chlamydial infection.

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页码：578 / 582

页数：5

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