ELECTRON-MICROSCOPIC LOCALIZATION OF PYRUVATE-CARBOXYLASE IN RAT-LIVER AND SACCHAROMYCES-CEREVISIAE BY IMMUNOGOLD PROCEDURES

被引：36

作者：

ROHDE, M ^{[1
]}

LIM, F ^{[1
]}

WALLACE, JC ^{[1
]}

机构：

[1] UNIV ADELAIDE,DEPT BIOCHEM,ADELAIDE,SA 5001,AUSTRALIA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1991年 / 290卷 / 01期

关键词：

D O I：

10.1016/0003-9861(91)90608-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The intracellular location of pyruvate carboxylase (EC 6.4.1.1) in rat liver and Saccharomyces cerevisiae was investigated using the antibody-gold and protein A-gold techniques carried out as a postembedding immunoelectron microscopic procedure. The vast majority of gold particles (> 98%), indicative of the presence of antigenic sites of pyruvate carboxylase, were found in the mitochondria of rat liver. No other cellular compartment was labeled except the cytosol which did not account for more than 2% of the total labeling of a rat hepatocyte. Furthermore, 60% of labeled pyruvate carboxylase molecules within a mitochondrion were found adjacent to the matrix side of the inner mitochondrial membrane. In contrast, in S. cerevisiae, pyruvate carboxylase was found exclusively in the cytosol. © 1991.

引用

页码：197 / 201

页数：5

共 35 条

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Barritt G.J., 1985, P141

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