NEUTROPHIL ALDOSE REDUCTASE-ACTIVITY AND ITS ASSOCIATION WITH ESTABLISHED DIABETIC MICROVASCULAR COMPLICATIONS

被引：37

作者：

DENT, MT ^{[1
]}

TEBBS, SE ^{[1
]}

GONZALEZ, AM ^{[1
]}

WARD, JD ^{[1
]}

WILSON, RM ^{[1
]}

机构：

[1] ROYAL HALLAMSHIRE HOSP,DEPT DIABET,FLOOR P,GLOSSOP RD,SHEFFIELD S10 2JF,S YORKSHIRE,ENGLAND

来源：

DIABETIC MEDICINE | 1991年 / 8卷 / 05期

关键词：

DIABETES; MICROVASCULAR COMPLICATIONS; ALDOSE REDUCTASE; POLYOL PATHWAY; NEUTROPHILS; SORBITOL DEHYDROGENASE;

D O I：

10.1111/j.1464-5491.1991.tb01628.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Direct investigation of the polyol pathway is rarely possible in studies of human diabetes. A spectrophotometric assay has been developed for the measurement of aldose reductase and sorbitol dehydrogenase activity in the neutrophil. Neutrophil aldose reductase activity was increased in patients with Type 1 diabetes with complications (median 40 (interquartile range 28-48) u, where 1 unit of enzyme activity = nmol NADPH min-1 10(8)-cells-1) compared with those without complications (20 (16-36) u, p < 0.01) and normal control subjects (20 (8-36) u, p < 0.01). In Type 2 diabetes, patients with complications also had higher aldose reductase activity (40 (28-52) u) than those without complications (24 (16-36) u, p < 0.01). There were no differences between patients without complications and normal control subjects. Sorbitol dehydrogenase activity was decreased in diabetic patients (p < 0.02) but not significantly different between diabetic patients with and without complications.

引用

页码：439 / 442

页数：4

共 26 条

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