NUCLEOTIDE-SEQUENCE OF A CARBOXYPHOSPHONOENOLPYRUVATE PHOSPHONOMUTASE GENE ISOLATED FROM A BIALAPHOS-PRODUCING ORGANISM, STREPTOMYCES-HYGROSCOPICUS, AND ITS EXPRESSION IN STREPTOMYCES-LIVIDANS

The carboxyphosphonoenolpyruvate (CPEP) phosphonomutase gene of bialaphos-producing Streptomyces hygroscopicus, which encodes a C-P bond forming enzyme was cloned into Streptomyces lividans and sequenced. The amino acid composition of the protein coded in an open reading frame of 295 codons and its calculated molecular mass, 32,800 Da, coincided well with those of the purified enzyme. Introduction of the CPEP phosphonomutase gene, the expression of which is controlled by the promoter of the aph gene, into S. lividans resulted in the production of this enzyme at a level almost equivalent to that in the parent strain.