DETECTION OF TOBACCO RATTLE VIRUS IN TUBERS EXHIBITING SYMPTOMS OF CORKY RINGSPOT BY POLYMERASE CHAIN-REACTION

被引：20

作者：

CROSSLIN, JM ^{[1
]}

THOMAS, PE ^{[1
]}

机构：

[1] USDA ARS,PROSSER,WA 99350

来源：

AMERICAN POTATO JOURNAL | 1995年 / 72卷 / 10期

关键词：

RT-PCR;

D O I：

10.1007/BF02849133

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Tobacco rattle virus (TRV)-specific products were obtained by reverse transcription-polymerase chain reaction (RT-PCR) of nucleic acid extracts prepared from tubers exhibiting symptoms of corky ringspot (CRS) disease, Viral origin of amplification products was confirmed by hybridization of PCR products with a nonradioactively-labeled complementary DNA probe prepared from purified TRV RNA. Results suggest that TRV is not uniformly distributed in CRS-affected tubers and appears to be in low concentration in tuber tissue.

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收藏

页码：605 / 609

页数：5

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