CONTINUOUS FRACTIONATION OF PROTEIN MIXTURES BY PH PARAMETRIC PUMPING - EXPERIMENT

被引：16

作者：

CHEN, HT

WONG, YW

WU, S

机构：

[1] Department of Chemical Engineering, New Jersey Institute of Technology, Newark, New Jersey

来源：

AICHE JOURNAL | 1979年 / 25卷 / 02期

关键词：

D O I：

10.1002/aic.690250215

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Continuous pH parametric pumping separations of a haemoglobin‐albumin‐CM Sepharose system were experimentally investigated. The parapump has a feed containing the protein mixture to be separated, introduced alternately to the top and bottom of the chromatographic column. The top and bottom products are withdrawn from the apparatus, respectively, during the bottom and top feed. It is shown that under certain conditions the pH driven parametric pump has the capacity for removal of protein components from one product fraction and large enrichment in the other fraction. Moreover, the continuous process can be operated with a large feed throughput. Copyright © 1979 American Institute of Chemical Engineers

引用

页码：320 / 327

页数：8

共 6 条

[1]

BRADLEY W, 1973, THESIS PRINCETON U

[2] PH CYCLING ZONE SEPARATION OF SUGARS - PREPARATIVE SEPARATION TECHNIQUE FOR COUNTERCURRENT DISTRIBUTION AND CHROMATOGRAPHY [J].

BUSBICE, ME ;

WANKAT, PC .

JOURNAL OF CHROMATOGRAPHY, 1975, 114 (02) :369-381

[3] SEPARATION OF PROTEINS VIA SEMICONTINUOUS PH PARAMETRIC PUMPING [J].

CHEN, HT ;

HSIEH, TK ;

LEE, HC ;

HILL, FB .

AICHE JOURNAL, 1977, 23 (05) :695-701

[4] PARAMETRIC PUMPING WITH PH [J].

SABADELL, JE ;

SWEED, NH .

SEPARATION SCIENCE, 1970, 5 (03) :171-&

[5] ENZYME SEPARATION BY PARAMETRIC PUMPING [J].

SHAFFER, AG ;

HAMRIN, CE .

AICHE JOURNAL, 1975, 21 (04) :782-786

[6]

1976, DEAE SEPHAROSE CL6B

← 1 →