PROPERTIES OF ALKALINE-PHOSPHATASE OF THE HUMAN DENTAL-PULP

被引：37

作者：

GOSEKI, M ^{[1
]}

OIDA, S ^{[1
]}

NIFUJI, A ^{[1
]}

SASAKI, S ^{[1
]}

机构：

[1] TOKYO MED & DENT UNIV, SCH DENT, DEPT ORAL SURG 1, BUNKYO KU, TOKYO 113, JAPAN

来源：

JOURNAL OF DENTAL RESEARCH | 1990年 / 69卷 / 03期

关键词：

D O I：

10.1177/00220345900690031601

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Enzymatic and immunological properties of alkaline phosphatase [ALP; orthophosphoric monoester phosphohydrolase, alkaline optimum, EC 3.1.3.1.] in the human dental pulp were investigated. In inhibition and thermal inactivation studies, dental pulp ALP showed properties of universal-type ALP (kidney/bone/liver type). Dental pulp ALP cross-reacted with polyclonal and monoclonal antibodies against purified swine-kidney ALP, and with monoclonal antibody against ALP of human osteoblast-like cells in the same manner as ALPs of human bone and kidney. The sodium dodecyl sulfate-gel electrophoretic pattern showed a 140, 000-Mr native protein band. These data suggest that dental pulp ALP can be classified as a universal-type ALP having antigenic determinants common to ALP of the kidney and bone. © 1990, SAGE Publications. All rights reserved.

引用

页码：909 / 912

页数：4

共 26 条

[1] STRUCTURAL EVIDENCE THAT HUMAN LIVER AND PLACENTAL ALKALINE-PHOSPHATASE ISOENZYMES ARE CODED BY DIFFERENT GENES [J].

BADGER, KS ;

SUSSMAN, HH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (07) :2201-2205

[2] PLACENTAL ALKALINE-PHOSPHATASE IN HOMINIDAE [J].

DOELLGAST, GJ ;

BENIRSCHKE, K .

NATURE, 1979, 280 (5723) :601-602

[3] HYDROLYTIC ENZYME HISTOCHEMISTRY OF RAT MOLAR PULP [J].

GOGGINS, JF ;

FULLMER, HM .

ARCHIVES OF ORAL BIOLOGY, 1967, 12 (05) :639-&

[4] EVOLUTION OF ALKALINE-PHOSPHATASES IN PRIMATES [J].

GOLDSTEIN, DJ ;

ROGERS, C ;

HARRIS, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (03) :879-883

[5] IMMUNOLOGICAL DIFFERENTIATION OF HUMAN UNIVERSAL TYPE ALKALINE-PHOSPHATASES BY A MONOCLONAL-ANTIBODY TO THE HUMAN-BONE CELL ENZYME [J].

GOSEKI, M ;

OIDA, S ;

OGATA, Y ;

SASAKI, S .

CLINICA CHIMICA ACTA, 1989, 180 (02) :189-191

[6] DETECTION OF MINOR IMMUNOLOGICAL DIFFERENCES AMONG HUMAN UNIVERSAL-TYPE ALKALINE-PHOSPHATASES [J].

GOSEKI, M ;

OIDA, S ;

SASAKI, S .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1988, 38 (03) :155-163

[7] BIOCHEMICAL STUDY OF ALKALINE-PHOSPHATASE IN ISOLATED RAT INCISOR ODONTOBLASTS [J].

GRANSTROM, G ;

LINDE, A .

ARCHIVES OF ORAL BIOLOGY, 1972, 17 (08) :1213-+

[8] PURIFICATION AND PROPERTIES OF BOVINE DENTAL-PULP ALKALINE-PHOSPHATASE [J].

HARADA, M ;

HIRAOKA, BY ;

FUKASAWA, K ;

FUKASAWA, KM .

ARCHIVES OF ORAL BIOLOGY, 1982, 27 (01) :69-74

[9]

HAWKES R, 1982, ANAL BIOCHEM, V119, P142, DOI 10.1016/0003-2697(82)90677-7

[10] NUCLEOTIDE AND AMINO-ACID-SEQUENCES OF HUMAN INTESTINAL ALKALINE-PHOSPHATASE - CLOSE HOMOLOGY TO PLACENTAL ALKALINE-PHOSPHATASE [J].

HENTHORN, PS ;

RADUCHA, M ;

EDWARDS, YH ;

WEISS, MJ ;

SLAUGHTER, C ;

LAFFERTY, MA ;

HARRIS, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (05) :1234-1238

← 1 2 3 →