ACTIVATED T-CELLS TRANSCRIBE AN ALTERNATIVELY SPLICED MESSENGER-RNA ENCODING A SOLUBLE FORM OF QA-2 ANTIGEN

被引:47
作者
ULKER, N [1 ]
LEWIS, KD [1 ]
HOOD, LE [1 ]
STROYNOWSKI, I [1 ]
机构
[1] CALTECH,DIV BIOL,147-75,PASADENA,CA 91125
关键词
activated T cells; alternative splicing; major histocompatibility complex; Qa-2; molecules;
D O I
10.1002/j.1460-2075.1990.tb07602.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Among the best characterized non-classical mouse major histocompatibility antigens are the Qa-2 molecules. These proteins can serve as targets for allogenic cytotoxic T cells and as signal transducing molecules. They are structurally similar to H-2 transplantation antigens in their N-terminal and β2-microglobulin binding domains but differ at their C-termini. While the H-2 antigens span the cell membrane, the Qa-2 molecules are attached to the cell surface via phospholipid anchors. The genetic information encoding this attachment is contained in exon 5. In concanavalin A activated splenocytes the expression of membrane bound Qa-2 antigens declines and, simultaneously, soluble forms of Qa-2 molecules are secreted. We demonstrate here that the soluble Qa-2 polypeptides are translated from alternatively spliced mRNAs lacking exon 5, while the membrane forms are encoded by the full-size transcripts. In cultured cells the alternative splicing of the Qa-2-message is induced by T-cell activation with concanavalin A. The canonical mRNA encoding the membrane form of Qa-2 predominates in unstimulated mouse tissues but the cultured cell lines, like activated T cells, express enhanced levels of the truncated mRNA. In some cell lines almost all Qa-2 transcripts lack exon 5. For example, in L cells, mRNAs encoding soluble Qa-2 molecules are at least 10 times more abundant than Qa-2 transcripts encoding phospholipid anchored antigens. These findings are discussed in terms of potential functions of membrane bound and secreted Qa-2 molecules.
引用
收藏
页码:3839 / 3847
页数:9
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