IMPROVED PROTECTION OF SWINE FROM PLEUROPNEUMONIA BY VACCINATION WITH PROTEINASE K-TREATED OUTER-MEMBRANE OF ACTINOBACILLUS (HAEMOPHILUS) PLEUROPNEUMONIAE

被引:18
作者
CHIANG, YW [1 ]
YOUNG, TF [1 ]
RAPPGABRIELSON, VJ [1 ]
ROSS, RF [1 ]
机构
[1] IOWA STATE UNIV SCI & TECHNOL, COLL VET MED, VET MED RES INST, AMES, IA 50011 USA
关键词
D O I
10.1016/0378-1135(91)90062-K
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The immunogenic and protective potentials of an outer membrane-enriched fraction (OM) from a serotype 5 strain of Actinobacillus (Haemophilus) pleuropneumoniae (APP) and the same OM degraded with proteinase K or periodate were evaluated in swine. Groups of pigs were vaccinated with two doses of OM, proteinase K-treated OM (P-OM), periodate-treated OM (PI-OM), or placebo vaccine and challenged intranasally with the homologous strain of APP. Results from triplicate experiments indicated that proteinase K treatment of OM resulted in an improved efficacy. This improved efficacy of P-OM vaccine over untreated OM vaccine was evidenced not only by less severe lung lesions in P-OM vaccinated pigs but also by significant reduction (P < 0.05) in the number of P-OM vaccinated pigs which developed lung lesions upon challenge with APP. Assessment of sera from vaccinated animals by immunoblotting, complement fixation test, or ELISA indicated that the immunogenicity of some but not all protein or carbohydrate components were reduced (or eliminated) by proteinase K and periodate treatments respectively.
引用
收藏
页码:49 / 62
页数:14
相关论文
共 35 条
[1]   TOXICITY OF HEMOPHILUS-PLEUROPNEUMONIAE FOR PORCINE LUNG MACROPHAGES, PERIPHERAL-BLOOD MONOCYTES, AND TESTICULAR CELLS [J].
BENDIXEN, PH ;
SHEWEN, PE ;
ROSENDAL, S ;
WILKIE, BN .
INFECTION AND IMMUNITY, 1981, 33 (03) :673-676
[2]  
BOSSE JT, 1990, P INT PIG VET SOC, V11, P8
[3]  
CHIANG YW, 1989, 70TH P ANN M C RES W, P58
[4]   MECHANISMS INVOLVED IN PROTECTION PROVIDED BY IMMUNIZATION AGAINST CORE LIPOPOLYSACCHARIDES OF ESCHERICHIA-COLI J5 FROM LETHAL HAEMOPHILUS-PLEUROPNEUMONIAE INFECTIONS IN SWINE [J].
FENWICK, BW ;
CULLOR, JS ;
OSBURN, BI ;
OLANDER, HJ .
INFECTION AND IMMUNITY, 1986, 53 (02) :298-304
[5]  
FENWICK BW, 1986, AM J VET RES, V47, P1888
[6]  
FENWICK BW, 1986, AM J VET RES, V47, P1433
[7]  
FREY J, 1989, JUN P INT C HAEM ACT, P41
[8]  
HIGGINS R, 1985, CAN VET J, V26, P86
[9]   MORPHOLOGICAL HETEROGENEITY AMONG SALMONELLA LIPOPOLYSACCHARIDE CHEMOTYPES IN SILVER-STAINED POLYACRYLAMIDE GELS [J].
HITCHCOCK, PJ ;
BROWN, TM .
JOURNAL OF BACTERIOLOGY, 1983, 154 (01) :269-277
[10]   VIRULENCE PROPERTIES AND PROTECTIVE EFFICACY OF THE CAPSULAR POLYMER OF HAEMOPHILUS-(ACTINOBACILLUS)-PLEUROPNEUMONIAE SEROTYPE-5 [J].
INZANA, TJ ;
MA, JN ;
WORKMAN, T ;
GOGOLEWSKI, RP ;
ANDERSON, P .
INFECTION AND IMMUNITY, 1988, 56 (08) :1880-1889