CLONED PLATELET THROMBIN RECEPTOR IS NECESSARY FOR THROMBIN-INDUCED PLATELET ACTIVATION

被引:182
作者
HUNG, DT
VU, TKH
WHEATON, VI
ISHII, K
COUGHLIN, SR
机构
[1] UNIV CALIF SAN FRANCISCO, CARDIOVASC RES INST, BOX 0524, HSW 846, SAN FRANCISCO, CA 94143 USA
[2] UNIV CALIF SAN FRANCISCO, DEPT LAB MED, SAN FRANCISCO, CA 94143 USA
[3] UNIV CALIF SAN FRANCISCO, DEPT MED, SAN FRANCISCO, CA 94143 USA
关键词
ANTIBODY; INHIBITOR; SERINE PROTEASES; THROMBOSIS; HIRUDIN;
D O I
10.1172/JCI115721
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Platelet activation by thrombin is critical for hemostasis and thrombosis. Structure-function studies with a recently cloned platelet thrombin receptor suggest that a hirudin-like domain in the receptor's extracellular amino terminal extension is a thrombin-binding determinant important for receptor activation. We now report that a peptide antiserum to this domain is a potent and specific antagonist of thrombin-induced platelet activation. This study demonstrates that the cloned platelet thrombin receptor is necessary for platelet activation by thrombin, and provides a strategy for developing blocking monoclonal antibodies of potential therapeutic value.
引用
收藏
页码:1350 / 1353
页数:4
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