Platelet activation by thrombin is critical for hemostasis and thrombosis. Structure-function studies with a recently cloned platelet thrombin receptor suggest that a hirudin-like domain in the receptor's extracellular amino terminal extension is a thrombin-binding determinant important for receptor activation. We now report that a peptide antiserum to this domain is a potent and specific antagonist of thrombin-induced platelet activation. This study demonstrates that the cloned platelet thrombin receptor is necessary for platelet activation by thrombin, and provides a strategy for developing blocking monoclonal antibodies of potential therapeutic value.
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页码:1350 / 1353
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Baenziger N L, 1974, Methods Enzymol, V31, P149
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SCRIPPS CLIN & RES FDN, RES INST, DEPT BASIC & CLIN RES, LA JOLLA, CA 92037 USASCRIPPS CLIN & RES FDN, RES INST, DEPT BASIC & CLIN RES, LA JOLLA, CA 92037 USA
HANSON, SR
HARKER, LA
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SCRIPPS CLIN & RES FDN, RES INST, DEPT BASIC & CLIN RES, LA JOLLA, CA 92037 USASCRIPPS CLIN & RES FDN, RES INST, DEPT BASIC & CLIN RES, LA JOLLA, CA 92037 USA
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SCRIPPS CLIN & RES FDN, RES INST, DEPT BASIC & CLIN RES, LA JOLLA, CA 92037 USASCRIPPS CLIN & RES FDN, RES INST, DEPT BASIC & CLIN RES, LA JOLLA, CA 92037 USA
HANSON, SR
HARKER, LA
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SCRIPPS CLIN & RES FDN, RES INST, DEPT BASIC & CLIN RES, LA JOLLA, CA 92037 USASCRIPPS CLIN & RES FDN, RES INST, DEPT BASIC & CLIN RES, LA JOLLA, CA 92037 USA