STABILITY AND DETECTION OF RECOMBINANT PRE-PRO-CONCANAVALIN-A AFTER CYTOPLASMIC EXPRESSION IN ESCHERICHIA-COLI

被引：11

作者：

WANG, M ^{[1
]}

JONES, DH ^{[1
]}

机构：

[1] UNIV COLL SWANSEA, SCH BIOL SCI, MOLEC BIOL RES GRP, SWANSEA SA2 8PP, W GLAM, WALES

来源：

FEBS LETTERS | 1992年 / 301卷 / 03期

关键词：

CONCANAVALIN-A; LEGUME LECTIN; POSTTRANSLATIONAL PROCESSING; RECOMBINANT PROTEIN STABILITY; TRANSCRIPTION TERMINATOR; WESTERN BLOTTING; CANAVALIA-ENSIFORMIS; ESCHERICHIA-COLI;

D O I：

10.1016/0014-5793(92)80265-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA for pre-pro-concanavalin A (pre-pro-Con A) from Canavalia ensiformis was used to construct two cytoplasmic expression vectors: pKconA (no transcription terminator) and pTKconA (containing the cry transcription terminator). The latter produced 2- to 3-fold greater amounts of pre-pro-Con A. This product containing the plant signal can be detected by Western blotting only after electrophoretic transfer in the presence of SDS, indicating reduced solubility. The signal is not removed and pre-pro-Con A is clearly stable after expression in E. coli JM109. The protein is not cleaved and ligated as in the plant, in contrast to a recent report.

引用

页码：315 / 318

页数：4

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