A SIMPLE METHOD FOR CONTINUOUS MEASUREMENT OF CELL HEIGHT DURING A VOLUME CHANGE IN A SINGLE A6 CELL

被引:12
作者
KAWAHARA, K
ONODERA, M
FUKUDA, Y
机构
[1] Department of Physiology, Chiba University School of Medicine, Chiba
关键词
A6; CELL; HYPOTONIC CELL SWELLING; PATCH-CLAMP EQUIPMENT;
D O I
10.2170/jjphysiol.44.411
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
A simple electrophysiological method for continuous monitoring of the height of a single renal cell. in culture is described. The procedure consists of placing a patch-electrode on the apical cell surface and monitoring the current deflections during osmotic challenge. The mean increase in cell height by 78% hypotonic solution was 2.0i+/-0.2 mu m.
引用
收藏
页码:411 / 419
页数:9
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