TRANSVERSE PORE GRADIENT GEL-ELECTROPHORESIS, USING THE PHASTSYSTEM

被引：4

作者：

BUZAS, Z

WHEELER, DL

GARNER, MM

TIETZ, D

CHRAMBACH, A

机构：

[1] NICHHD,THEORET & PHYS BIOL LAB,MACROMOLEC ANAL SECT,BETHESDA,MD 20892

[2] AGR BIOTECHNOL CTR,INST BIOCHEM & PROT RES,H-2101 GODOLLO,HUNGARY

[3] NIDDKD,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD

[4] UNIV GIESSEN,W-6300 GIESSEN,GERMANY

来源：

ELECTROPHORESIS | 1994年 / 15卷 / 8-9期

关键词：

D O I：

10.1002/elps.11501501153

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of pore gradient gels prefabricated for the PhastSystem (Pharmacia) to transverse pore gradient gel electrophoresis is demonstrated. It has the twofold advantage of (i) horizontal positioning, avoiding gel stretching during the preparation of these gels and resulting pore size irreproducibility experienced with vertically applied pore gradient gels, which necessitate an orthogonal transfer of spacers, and (ii) miniaturized gel dimensions, which allow a small sample load and a short duration of electrophoresis and staining.

引用

页码：1028 / 1031

页数：4

共 13 条

[1]

CHRAMBACH A, 1994, ELECTROPHORESIS, V15

[2] TRANSVERSE AGAROSE PORE GRADIENT GEL-ELECTROPHORESIS OF DNA [J].