THE SSS GENE-PRODUCT, WHICH AFFECTS PYOVERDIN PRODUCTION IN PSEUDOMONAS-AERUGINOSA 7NSK2, IS A SITE-SPECIFIC RECOMBINASE

被引：48

作者：

HOFTE, M

DONG, QG

KOURAMBAS, S

KRISHNAPILLAI, V

SHERRATT, D

MERGEAY, M

机构：

[1] VLAAMSE INSTELLING TECHNOL ONDERZOEK, GENET & BIOTECHNOL LAB, B-2400 MOL, BELGIUM

[2] STATE UNIV GHENT, PHYTOPATHOL LAB, B-9000 GHENT, BELGIUM

[3] MONASH UNIV, DEPT GENET & DEV BIOL, CLAYTON, VIC 3168, AUSTRALIA

[4] UNIV OXFORD, DEPT BIOCHEM, OXFORD OX1 3QU, ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1994年 / 14卷 / 05期

关键词：

D O I：

10.1111/j.1365-2958.1994.tb01335.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pyoverdin production by Pseodomonas aeruginosa strain 7NSK2 was induced by Zn(ll) in the presence of iron. A mutant was isolated in which Zn(ll) no longer induced pyoverdin production. The sss gene which was inactivated in this mutant was cloned and sequenced. Its protein sequence showed 50% identity to the XerC protein of Escherichia coli, which is a member of the lambda integrase family of site-specific recombinases, An open reading frame was found upstream of sss whose protein sequence showed strong identity to DapF, the diaminopimelate epimerase. In E. coli, xerC is part of a multicistronic unit that also contains dapF, The sss gene of P. aeruginosa could restore site-specific recombination at cer in an E. coli xerC mutant and the E. coli xerC gene could complement a genomic sss mutation in P. aeruginosa.

引用

收藏

页码：1011 / 1020

页数：10

相关论文

共 47 条

[1] MAPPING OF MUTATIONS IN PSEUDOMONAS-AERUGINOSA DEFECTIVE IN PYOVERDIN PRODUCTION [J].

ANKENBAUER, R ;

HANNE, LF ;

COX, CD .

JOURNAL OF BACTERIOLOGY, 1986, 167 (01) :7-11

[2] THE INTEGRASE FAMILY OF SITE-SPECIFIC RECOMBINASES - REGIONAL SIMILARITIES AND GLOBAL DIVERSITY [J].

ARGOS, P ;

LANDY, A ;

ABREMSKI, K ;

EGAN, JB ;

HAGGARDLJUNGQUIST, E ;

HOESS, RH ;

KAHN, ML ;

KALIONIS, B ;

NARAYANA, SVL ;

PIERSON, LS ;

STERNBERG, N ;

LEONG, JM .

EMBO JOURNAL, 1986, 5 (02) :433-440

[3] SPECIFIC-PURPOSE PLASMID CLONING VECTORS .2. BROAD HOST RANGE, HIGH COPY NUMBER, RSF1010-DERIVED VECTORS, AND A HOST-VECTOR SYSTEM FOR GENE CLONING IN PSEUDOMONAS [J].

BAGDASARIAN, M ;

LURZ, R ;

RUCKERT, B ;

FRANKLIN, FCH ;

BAGDASARIAN, MM ;

FREY, J ;

TIMMIS, KN .

GENE, 1981, 16 (1-3) :237-247

[4] ACTIVITY OF THE HYBRID TRP-LAC(TAC) PROMOTER OF ESCHERICHIA-COLI IN PSEUDOMONAS-PUTIDA - CONSTRUCTION OF BROAD-HOST-RANGE, CONTROLLED-EXPRESSION VECTORS [J].

BAGDASARIAN, MM ;

AMANN, E ;

LURZ, R ;

RUCKERT, B ;

BAGDASARIAN, M .

GENE, 1983, 26 (2-3) :273-282

[5] FERRIC UPTAKE REGULATION PROTEIN ACTS AS A REPRESSOR, EMPLOYING IRON(II) AS A COFACTOR TO BIND THE OPERATOR OF AN IRON TRANSPORT OPERON IN ESCHERICHIA-COLI [J].

BAGG, A ;

NEILANDS, JB .

BIOCHEMISTRY, 1987, 26 (17) :5471-5477

[6]

BLAKELY G, 1991, NEW BIOL, V3, P789

[7] 2 RELATED RECOMBINASES ARE REQUIRED FOR SITE-SPECIFIC RECOMBINATION AT DIF AND CER IN ESCHERICHIA-COLI K12 [J].

BLAKELY, G ;

MAY, G ;

MCCULLOCH, R ;

ARCISZEWSKA, LK ;

BURKE, M ;

LOVETT, ST ;

SHERRATT, DJ .

CELL, 1993, 75 (02) :351-361

[8] A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].

BOYER, HW ;

ROULLAND.D .

JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) :459-&

[9] EFFECT OF GROWTH CONDITIONS ON FORMATION OF RELAXATION COMPLEX OF SUPERCOILED COLE1 DEOXYRIBONUCLEIC ACID AND PROTEIN IN ESCHERICHIA-COLI [J].

CLEWELL, DB ;

HELINSKI, DR .

JOURNAL OF BACTERIOLOGY, 1972, 110 (03) :1135-&

[10] RECOMBINATION AT COLE1 CER REQUIRES THE ESCHERICHIA-COLI XERC GENE-PRODUCT, A MEMBER OF THE LAMBDA-INTEGRASE FAMILY OF SITE-SPECIFIC RECOMBINASES [J].

COLLOMS, SD ;

SYKORA, P ;

SZATMARI, G ;

SHERRATT, DJ .

JOURNAL OF BACTERIOLOGY, 1990, 172 (12) :6973-6980

← 1 2 3 4 5 →