HUMAN AUTOANTIBODIES TO THE THYROTROPIN RECEPTOR - RECOGNITION OF LINEAR, FOLDED, AND GLYCOSYLATED RECOMBINANT EXTRACELLULAR DOMAIN

被引：45

作者：

VLASE, H

GRAVES, PN

MAGNUSSON, RP

DAVIES, TF

机构：

[1] CUNY MT SINAI SCH MED, DEPT MED, DIV ENDOCRINOL & METAB, NEW YORK, NY 10029 USA

[2] CUNY MT SINAI SCH MED, DEPT PHARMACOL, NEW YORK, NY 10029 USA

来源：

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | 1995年 / 80卷 / 01期

关键词：

D O I：

10.1210/jc.80.1.46

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

To examine the heterogeneity of autoantibodies to the human TSH receptor (hTSHR), we evaluated 20 sera from patients with Graves' disease for their recognition of prokaryotic (unglycosylated) and eukaryotic (insect cell glycosylated) recombinant hTSHR extracellular domain (ecd) in an unfolded (linear) and a folded (nonlinear) state. With the prokaryotic antigen, 12 (60%) bound folded hTSHR ecd monomer, 8 (40%) bound to the unfolded monomer, and 3 (15%) bound to a tetrameric species. Such binding to different hTSHR antigens was not mutually exclusive. In addition, 7 (35%) sera showed an apparently higher reactivity for the folded than the unfolded monomer.

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页码：46 / 53

页数：8

相关论文

共 23 条

[1] HIGH EXPRESSION OF THE HORMONE BINDING ACTIVE EXTRACELLULAR DOMAIN (1-294) OF RAT LUTROPIN RECEPTOR IN ESCHERICHIA-COLI [J].

CHEN, WY ;

BAHL, OP .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1993, 91 (1-2) :35-41

[2] THE SIZE OF THE MATURE MEMBRANE-RECEPTOR FOR FOLLICLE-STIMULATING-HORMONE IS LARGER THAN THAT PREDICTED FROM ITS CDNA [J].

DATTATREYAMURTY, B ;

SMITH, RA ;

ZHANG, SB ;

SANTACOLOMA, TA ;

REICHERT, LE .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 1992, 9 (02) :115-121

[3] EVIDENCE OF LIMITED VARIABILITY OF ANTIGEN RECEPTORS ON INTRATHYROIDAL T-CELLS IN AUTOIMMUNE THYROID-DISEASE [J].

DAVIES, TF ;

MARTIN, A ;

CONCEPCION, ES ;

GRAVES, P ;

COHEN, L ;

BENNUN, A .

NEW ENGLAND JOURNAL OF MEDICINE, 1991, 325 (04) :238-244

[4] ANALYSIS OF AUTOANTIBODY REACTIVITY IN PATIENTS WITH GRAVES-DISEASE USING RECOMBINANT EXTRACELLULAR DOMAIN OF THE HUMAN THYROTROPIN RECEPTOR AND SYNTHETIC PEPTIDES [J].

FAN, JL ;

SEETHARAMAIAH, GS ;

DESAI, RK ;

DALLAS, JS ;

WAGLE, NM ;

PRABHAKAR, BS .

AUTOIMMUNITY, 1993, 15 (04) :285-291

[5]

GRAVES PN, 1995, IN PRESS ENDOCRINOLO

[6] ELUTION OF PROTEINS FROM SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS, REMOVAL OF SODIUM DODECYL-SULFATE, AND RENATURATION OF ENZYMATIC-ACTIVITY - RESULTS WITH SIGMA SUBUNIT OF ESCHERICHIA-COLI RNA-POLYMERASE, WHEAT-GERM DNA TOPOISOMERASE, AND OTHER ENZYMES [J].

HAGER, DA ;

BURGESS, RR .

ANALYTICAL BIOCHEMISTRY, 1980, 109 (01) :76-86

[7]

HARLOW E, 1988, ANTIBODIES LABORATOR

[8] EXPRESSION OF A HUMAN THYROTROPIN RECEPTOR FRAGMENT IN ESCHERICHIA-COLI AND ITS INTERACTION WITH THE HORMONE AND AUTOANTIBODIES FROM PATIENTS WITH GRAVES-DISEASE [J].

HUANG, GC ;

COLLISON, KS ;

MCGREGOR, AM ;

BANGA, JP .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 1992, 8 (02) :137-144

[9] THE THYROTROPIN HORMONE RECEPTOR OF GRAVES-DISEASE - OVEREXPRESSION OF THE EXTRACELLULAR DOMAIN IN INSECT CELLS USING RECOMBINANT BACULOVIRUS, IMMUNOAFFINITY PURIFICATION AND ANALYSIS OF AUTOANTIBODY BINDING [J].

HUANG, GC ;

PAGE, MJ ;

NICHOLSON, LB ;

COLLISON, KS ;

MCGREGOR, AM ;

BANGA, JP .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 1993, 10 (02) :127-142

[10] EXPRESSION OF HUMAN THYROID PEROXIDASE IN INSECT CELLS USING RECOMBINANT BACULOVIRUS [J].

KENDLER, DL ;

BRENNAN, V ;

DAVIES, TF ;

MAGNUSSON, RP .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1993, 93 (02) :199-206