PRESERVATION BY GLYCEROL OF DNA SYNTHETIC CAPACITY OF ISOLATED HELA S3 CELL-NUCLEI DURING LONG-TERM STORAGE AT LOW-TEMPERATURE

被引：13

作者：

WIST, E

KROKAN, H

机构：

[1] Institute of Medical Biology, University of Tromsø

来源：

EXPERIMENTAL CELL RESEARCH | 1978年 / 116卷 / 02期

关键词：

D O I：

10.1016/0014-4827(78)90453-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A method for storage of nuclei which preserves the DNA synthetic activity is presented. Freezing of nuclei in a hypotonic buffer containing 70% glycerol preserves fully the DNA synthetic capacity. DNA synthesis continues at sites that were active in vivo and proceeds through synthesis of early nascent intermediates (primary DNA pieces) which are subsequently joined into DNA of larger molecular weight. © 1978.

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页码：313 / 316

页数：4

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