MODULATION OF CONCANAVALIN A STIMULATION OF HAMSTER LYMPHOID-CELLS BY LITHIUM-CHLORIDE

被引：25

作者：

HART, DA

机构：

[1] Department of Microbiology, University of Texas Health Science Center at Dallas, Dallas

来源：

CELLULAR IMMUNOLOGY | 1979年 / 43卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0008-8749(79)90155-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Addition of LiCl (1-25 mM) to serum-free cultures of MHA hamster thymocytes, lymph node cells, or splenocytes stimulated with concanavalin A had a biphasic effect on [3H]thymidine incorporation. These concentrations of LiCl enhanced stimulation of [3H]thymidine incorporation by suboptimal levels of concanavalin A but inhibited stimulation of optimal and supraoptimal concentrations of concanavalin A. This effect was specific for Li+ since it was not observed when similar concentrations of Na+, K+, or Mg2+ were added to cultures stimulated by concanavalin A. The inhibitory effect of LiCl on concanavalin A stimulation was not reversed by addition of Na+, Ca2+, Mg2+, or Ca2+ + Mg2+ to the cultures. Significant reversal of LiCl inhibition of stimulation was observed when KCl was added to the cultures. However none of the ions tested blocked the Li-induced enhancement of [3H]thymidine incorporation in the presence of suboptimal concentrations of concanavalin A. © 1979.

引用

页码：113 / 122

页数：10

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