IMPROVED PROCEDURE FOR SOLUBILIZATION AND ASSAY OF BLOOD AND FECES IN LIQUID SCINTILLATION-COUNTING

被引：15

作者：

MICHAELS, EB

HAHN, EC

KENYON, AJ

机构：

[1] Memorial Sloan-Kettering Cancer Center, Walker Laboratory, Rye, NY 10580

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 99卷 / 02期

关键词：

D O I：

10.1016/S0003-2697(79)80009-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new procedure for solubilization of blood and feces using ammonium bicarbonate in the presence of Triton X-100 and trypsin with peroxide decolorization was tested and compared to conventional methods. The procedure gave two- to fourfold increases of efficiency and lower backgrounds approaching that of the cocktail alone within 2-4 h. Recovery of tritium radioactivity is equivalent to recovery by dry oxidation. © 1979 Academic Press, Inc.

引用

页码：288 / 296

页数：9

共 17 条

[1]

[Anonymous], 1976, INTRO LINEAR REGRESS

[2]

BRANSOME ED, 1970, LIQUID SCINTILLATION, P342

[3] DECOLORIZATION OF FECAL EXTRACTS FOR RADIOACTIVE ASSAY [J].