POLYAMINE CONTENT OF A NON-AQUEOUSLY ISOLATED CHROMOSOME PREPARATION

被引：15

作者：

GOYNS, MH ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT ZOOL,CAMBRIDGE CB2 3EL,ENGLAND

来源：

EXPERIMENTAL CELL RESEARCH | 1979年 / 122卷 / 02期

关键词：

D O I：

10.1016/0014-4827(79)90313-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Chromosomes were isolated from synchronized metaphase HeLa cells by a non-aqueous technique. Labelling of the cells with [14C]polyamine demonstrated that there is very little redistribution of polyamine from the cytoplasm using this method. The chromosomes were not obtained singly but were released from the cells in clusters. The polyamine content of these clusters was estimated as 135.9 pmoles spermine/μg DNA and 116.1 pmoles spermidine/μg DNA, producing a spermidine: spermine ratio of 1:1.17 as compared with a ratio of 1:0.67 for the whole cell. © 1979.

引用

页码：377 / 380

页数：4

共 29 条

[1] INTERACTIONS OF POLYAMINES AND ACID MACROMOLECULES OBSERVED IN DOUBLE DIFFUSION IN GEL EXPERIMENTS [J].