AN ENZYMATIC APPROACH TO THE ANALYSIS OF MICROBIAL ACTIVITY DURING PLANT LITTER DECOMPOSITION

被引:222
作者
SINSABAUGH, RL
ANTIBUS, RK
LINKINS, AE
机构
[1] Biology Department, Clarkson University, Potsdam
关键词
D O I
10.1016/0167-8809(91)90092-C
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The microbial degradation of plant litter is mediated by extracellular enzymes. Their specificity makes enzymes assays useful for comparing microbial communities, monitoring community succession, evaluating the effects of disturbance or ecosystem variables on microbial processes and for studying microbial processes at the molecular level. Thus far, the application of enzymic techniques to ecological studies of plant litter decomposition has been limited. Applied in combination with general indices of microbial biomass and activity, taxonomic analyses and process rate measurements, enzyme assays offer a mechanistic approach to decomposition studies. This potential is illustrated by discussing the role of cellulase in leaf litter degradation and by proposing a conceptual model of plant litter degradation from an enzymic perspective.
引用
收藏
页码:43 / 54
页数:12
相关论文
共 52 条
[1]   EXTRACTION, PURIFICATION AND PROPERTIES OF SOIL HYDROLASES [J].
BATISTIC, L ;
SARKAR, JM ;
MAYAUDON, J .
SOIL BIOLOGY & BIOCHEMISTRY, 1980, 12 (01) :59-63
[2]   INHIBITION OF DECOMPOSITION OF CELLULOSE AND SOME OTHER CARBOHYDRATES BY TANNIN [J].
BENOIT, RE ;
STARKEY, RL .
SOIL SCIENCE, 1968, 105 (05) :291-&
[3]  
Bremner J.M., 1978, SOIL ENZYMES, P149
[4]  
BRUNS RG, 1978, SOIL ENZYMES, P295
[5]  
Burns R. G., 1983, Symposia of the Society for General Microbiology, P249
[6]  
CHAMIER AC, 1985, BOT J LINN SOC, V91, P67, DOI 10.1111/j.1095-8339.1985.tb01136.x
[7]   BACTERIAL BIOFILMS IN NATURE AND DISEASE [J].
COSTERTON, JW ;
CHENG, KJ ;
GEESEY, GG ;
LADD, TI ;
NICKEL, JC ;
DASGUPTA, M ;
MARRIE, TJ .
ANNUAL REVIEW OF MICROBIOLOGY, 1987, 41 :435-464
[8]  
COXON DS, 1987, SOIL BIOL BIOCHEM, V19, P49
[9]   OXIDATION - IMPORTANT ENZYME REACTION IN FUNGAL DEGRADATION OF CELLULOSE [J].
ERIKSSON, KE ;
PETTERSSON, B ;
WESTERMARK, U .
FEBS LETTERS, 1974, 49 (02) :282-285
[10]  
Eriksson KE, 1985, BIOSYNTHESIS BIODEGR, P469