DUAL ULTRAVIOLET WAVELENGTH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE FORENSIC OR CLINICAL ANALYSIS OF 17 ANTIDEPRESSANTS AND SOME SELECTED METABOLITES

被引：32

作者：

MCINTYRE, IM ^{[1
]}

KING, CV ^{[1
]}

SKAFIDIS, S ^{[1
]}

DRUMMER, OH ^{[1
]}

机构：

[1] MONASH UNIV,DEPT FORENS MED,S MELBOURNE,VIC 3205,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 621卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80098-O

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive method suitable for the determination of tricyclic and other antidepressants in postmortem and clinical specimens is presented. The procedure, which utilizes reversed-phase HPLC combined with dual ultraviolet wavelength detection, enables the separation of 17 commonly prescribed antidepressants and some selected metabolites in a single extraction. Peak purity was confirmed using absorbance ratios at 220 nm and 254 nm wavelengths and revealed little interference from other eluting analytes. The blood detection limit for most antidepressants was 50 ng/ml. The most commonly observed antidepressants in 281 forensic cases analysed over a two-year period with the described method were dothiepin, amitriptyline, nortriptyline and doxepin.

引用

页码：215 / 223

页数：9

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