ANTI-SYNTAXIN ANTIBODIES INHIBIT CALCIUM-DEPENDENT CATECHOLAMINE SECRETION FROM PERMEABILIZED CHROMAFFIN CELLS

被引：30

作者：

GUTIERREZ, LM

QUINTANAR, JL

VINIEGRA, S

SALINAS, E

MOYA, F

REIG, JA

机构：

[1] UNIV ALICANTE,FAC MED,DEPT NEUROQUIM,E-03080 ALACANT,SPAIN

[2] UNIV ALICANTE,FAC MED,DEPT GENET & MICROBIOL,E-03080 ALACANT,SPAIN

[3] UNIV ALICANTE,INST NEUROCIENCIAS,E-03080 ALACANT,SPAIN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 206卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adrenomedullary chromaffin cells release catecholamines in response to the intracellular calcium rise upon stimulation by different secretagogues. The presence of syntaxin 1, a protein presumably involved in docking of synaptic vesicles to presynaptic membranes, has been investigated in chromaffin cells. The study using two different monoclonal antibodies shows that syntaxin 1 is present in the chromaffin cell membrane fraction. Functional experiments demonstrate that anti-syntaxin antibodies inhibit calcium-dependent secretion in permeabilized cells. These results suggest that syntaxin 1 is an important component of the secretory machinery in chromaffin cells. (C) 1995 Academic Press, Inc.

引用

页码：1 / 7

页数：7

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