2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS (PAGE) SYSTEM USING SODIUM DODECYL SULFATE-PAGE IN THE 1ST DIMENSION

被引：26

作者：

TUSZYNSKI, GP ^{[1
]}

BUCK, CA ^{[1
]}

WARREN, L ^{[1
]}

机构：

[1] WISTAR INST ANAT & BIOL, PHILADELPHIA, PA 19104 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 93卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0003-2697(79)80159-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A two-dimensional gel electrophoretic system for the separation of cellular proteins is described. The system utilizes sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis in the first dimension and polyacrylamide gel isoelectric focusing in the second dimension. The system offers a good starting point for many difficult protein separations requiring SDS. © 1979 Academic Press, Inc.

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页码：329 / 338

页数：10

相关论文

共 24 条

[1] 2-DIMENSIONAL GEL-ELECTROPHORESIS OF MEMBRANE PROTEINS [J].

AMES, GFL ;

NIKAIDO, K .

BIOCHEMISTRY, 1976, 15 (03) :616-623

[2] CYTOSKELETAL ELEMENTS OF CHICK-EMBRYO FIBROBLASTS REVEALED BY DETERGENT EXTRACTION [J].

BROWN, S ;

LEVINSON, W ;

SPUDICH, JA .

JOURNAL OF SUPRAMOLECULAR STRUCTURE, 1976, 5 (02) :119-130

[3] A COMPARATIVE STUDY OF GLYCOPROTEINS FROM SURFACE OF CONTROL AND ROUS SARCOMA VIRUS TRANSFORMED HAMSTER CELLS [J].

BUCK, CA ;

GLICK, MC ;

WARREN, L .

BIOCHEMISTRY, 1970, 9 (23) :4567-&

[4] POLYACRYLAMIDE GEL ELECTROPHORESIS [J].

CHRAMBACH, A ;

RODBARD, D .

SCIENCE, 1971, 172 (3982) :440-+

[5]

CLARKE S, 1975, J BIOL CHEM, V250, P5459

[6] ISOELECTRIC-FOCUSING OF PROTEINS SEPARATED BY SDS-POLYACRYLAMIDE GEL-ELECTROPHORESIS [J].

DANNO, GI .

ANALYTICAL BIOCHEMISTRY, 1977, 83 (01) :189-193

[7] ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].

FAIRBANKS, G ;

STECK, TL ;

WALLACH, DFH .

BIOCHEMISTRY, 1971, 10 (13) :2606-+

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9] HETEROGENEITY OF MOUSE-CHROMATIN NONHISTONE PROTEINS AS EVIDENCED BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS AND ION-EXCHANGE CHROMATOGRAPHY [J].

MACGILLIVRAY, AJ ;

RICKWOOD, D .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 41 (01) :181-190

[10]

OFARRELL PH, 1975, J BIOL CHEM, V250, P4007