EFFECT OF SWIM-UP, PERCOLL AND SEPHADEX SPERM SEPARATION METHODS ON THE HYPOOSMOTIC SWELLING TEST

被引：14

作者：

CHECK, JH ^{[1
]}

KATSOFF, D ^{[1
]}

KOZAK, J ^{[1
]}

LURIE, D ^{[1
]}

机构：

[1] UNIV MED & DENT NEW JERSEY,COOPER HOSP,ROBERT WOOD JOHNSON MED SCH,MED CTR,DEPT OBSTET & GYNECOL,CAMDEN,NJ

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 01期

关键词：

HYPOOSMOTIC SWELLING TEST; PERCOL; SEPHADEX; SWIM-UP;

D O I：

10.1093/oxfordjournals.humrep.a137540

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The separation of spermatozoa from the seminal plasma is required to prepare the spermatozoa for intrauterine insemination, in-vitro fertilization and sex selection. This study evaluated the effects of sperm preparation techniques on the functional integrity of the sperm membrane, as measured by the hypo-osmotic swelling test (HOS). Thirty-four semen specimens obtained from the male partner of infertile couples were evaluated. A semen analysis and HOS test were performed on each specimen. The remainder of the specimen was divided into three equal aliquots, the first prepared using Percoll, the second using a swim-up method and the third using a Sephadex column. After the preparation, a semen analysis and HOS test was performed on each aliquot. The mean and standard deviation for the HOS test was 72.9 +/- 8.5% initially, 71.2 +/- 13.1 after Percoll, 75.2 +/- 15.1 after swim-up and 62.4 +/- 14.5 after Sephadex. Analysis of variance showed that the mean HOS score was the same after Percoll and swim-up as it was initially but significantly lower after preparation with Sephadex. There was also a higher proportion of abnormal semen specimens (HOS < 50%) after preparation with Sephadex than after the other preparation methods. We recommend the use of the HOS test as part of a screening panel for sperm separation.

引用

页码：109 / 111

页数：3

共 29 条

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