LOCALIZATION OF ANGIOTENSIN PEPTIDE-FORMING ENZYMES OF 3T3-F442A ADIPOCYTES

被引:68
作者
SAYE, JA
RAGSDALE, NV
CAREY, RM
PEACH, MJ
机构
[1] UNIV VIRGINIA, DEPT PHARMACOL, CHARLOTTESVILLE, VA 22908 USA
[2] UNIV VIRGINIA, DEPT INTERNAL MED, CHARLOTTESVILLE, VA 22908 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 06期
关键词
RENIN-LIKE ENZYMES; ANGIOTENSINOGEN; ANGIOTENSIN-I; ANGIOTENSIN-II;
D O I
10.1152/ajpcell.1993.264.6.C1570
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We have demonstrated that angiotensinogen is synthesized by 3T3-F442A cells and is hydrolyzed to angiotensins I and II (ANG I and II) by this model adipocyte system. This study was designed to determine whether ANG I is generated by renin or some other enzyme and where the formation of ANG I and/or II occurs in 3T3-F442A cells. Renin mRNA was not detected by Northern blot analysis of poly(A)+-selected RNA from cultures of fully differentiated adipocytes nor by the more sensitive polymerase chain reaction, implying that renin is not synthesized in this model adipocyte system. Hydrolysis of angiotensinogen to ANG I and II was demonstrated to be associated with the cell but not the media. Inhibitors, including EDTA, aimed at inactivating enzymes belonging to the serine, acid, or aspartyl proteases, and metalloproteases were ineffective in preventing the formation of either ANG I or II. Therefore the model adipocyte 3T3-F442A cell system forms ANG I and II in the absence of renin and angiotensin-converting enzyme. The unidentified enzymes responsible for peptide formation are associated with the cell itself.
引用
收藏
页码:C1570 / C1576
页数:7
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