THE 3A PROTEIN FROM CUCUMBER MOSAIC-VIRUS INCREASES THE GATING CAPACITY OF PLASMODESMATA IN TRANSGENIC TOBACCO PLANTS

被引:69
作者
VAQUERO, C
TURNER, AP
DEMANGEAT, G
SANZ, A
SERRA, MT
ROBERTS, K
GARCIALUQUE, I
机构
[1] CSIC,CTR INVEST BIOL,DEPT PLANT BIOL,E-28006 MADRID,SPAIN
[2] JOHN INNES INST,DEPT CELL BIOL,NORWICH NR4 7UH,NORFOLK,ENGLAND
[3] BIOCEM,F-63170 CLERMONT FERRAND,FRANCE
关键词
D O I
10.1099/0022-1317-75-11-3193
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The 3a protein, encoded by RNA 3 of cucumber mosaic virus (CMV), is the putative movement protein of viral progeny in infected plants. An analysis of transgenic tobacco plants constitutively expressing the CMV 3a protein showed that the protein is accumulated in leaves at every stage of development. In fully expanded leaves the protein is immunodetectable mostly in a cell-wall-enriched fraction. Dye-coupling experiments using fluorescent-dextran probes were performed on fully expanded leaves to study the modifying effect of CMV 3a protein on the gating capacity of plasmodesmata. Movement of fluorescein-isothiocyanate-labelled dextran with a mean molecular mass of 10000 Da, and an approximate Stokes' radius of 2.3 nm, was detected between cells of the 3a protein transgenic plants, but not in the control plants. These results are consistent with the idea that the CMV 3a protein is involved in the modification of plasmodesmata and, therefore, in the cell-to-cell spread of the virus.
引用
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页码:3193 / 3197
页数:5
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