OXALOACETATE DECARBOXYLASE FROM COD . A SHORTER PREPARATION AND CRYSTALLIZATION

被引：27

作者：

KOSICKI, GW

机构：

[1] James Bryant Conant Laboratory, Department of Chemistry, Harvard University, Cambridge

来源：

BIOCHEMISTRY | 1968年 / 7卷 / 12期

关键词：

D O I：

10.1021/bi00852a022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystalline oxaloacetate decarboxylase can be prepared from codfish muscle (Gadus marrhua) by the following steps: extraction of the frozen muscle, adjusting the pH to 5.1, heating to 45°, fractionation of the enzyme with (NH4)2SO4, chromatography over a Ca3(PO4)2-cellulose column, concentration, and crystallization; yield ~80-85 mg from 500 g of frozen muscle. Variations of this method are also presented. © 1968, American Chemical Society. All rights reserved.

引用

页码：4299 / &