Strain Aureobasidium pullulans capable of utilizing hemicelluloses and xylan was cultivated on processed waste dialysis liquor from the production of viscose fibres, containing about 1.5 % hemicelluloses. Basic conditions of biomass production were tested on a laboratory scale. The dialysis waste liquor adjusted with mineral acids to pH 4 - 5 and supplemented with 0.05 % yeast autolyzate and 0.2 % ammonium sulphate affords protein yields of about 0.8 g/1, corresponding to 4.0 - 4.5 g dry biomass. Biomass is isolated together with residual water-insoluble hemicelluloses which are not utilized by the microorganism. The total utilization of hemicelluloses attains about 70 %. © 1979 Institute of Microbiology, Academy of Sciences of the Czech Republic.