PURIFICATION AND PROPERTIES OF GLUTAMINE SYNTHETASES FROM THE CYANOBACTERIA SYNECHOCYSTIS SP STRAIN PCC-6803 AND CALOTHRIX SP STRAIN PCC-7601

被引:51
作者
MERIDA, A
LEURENTOP, L
CANDAU, P
FLORENCIO, FJ
机构
[1] UNIV SEVILLA,CSIC,INST BIOQUIM VEGETAL & FOTOSINTESIS,DEPT BIOQUIM VEGETAL & BIOL MOLEC,E-41080 SEVILLE,SPAIN
[2] UNIV INSTELLING ANTWERP,DEPT BIOL,B-2610 WILRIJK,BELGIUM
关键词
D O I
10.1128/jb.172.8.4732-4735.1990
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Glutamine synthetases (GSs) from two cyanobacteria, one unicellular (Synechocystis sp. strain PCC 6803) and the other filamentous (Calothrix sp. strain PCC 7601 [Fremyella diplosiphon]), were purified to homogeneity. The biosynthetic activities of both enzymes were strongly inhibited by ADP, indicating that the energy charge of the cell might regulate the GS activity. Both cyanobacteria exhibited an ammonium-mediated repression of GS synthesis. In addition, the Synechocystis sp. showed an inactivation of GS promoted by ammonium that had not been demonstrated previously in cyanobacteria.
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页码:4732 / 4735
页数:4
相关论文
共 23 条
[1]   NOVEL SUBUNIT SUBUNIT INTERACTIONS IN THE STRUCTURE OF GLUTAMINE-SYNTHETASE [J].
ALMASSY, RJ ;
JANSON, CA ;
HAMLIN, R ;
XUONG, NH ;
EISENBERG, D .
NATURE, 1986, 323 (6086) :304-309
[2]   PURIFICATION AND PROPERTIES OF GLUTAMINE-SYNTHETASE FROM THE NON-N2-FIXING CYANOBACTERIUM PHORMIDIUM-LAMINOSUM [J].
BLANCO, F ;
ALANA, A ;
LLAMA, MJ ;
SERRA, JL .
JOURNAL OF BACTERIOLOGY, 1989, 171 (02) :1158-1165
[3]  
DEUEL TF, 1970, J BIOL CHEM, V245, P5206
[4]   A CLONED CYANOBACTERIAL GENE FOR GLUTAMINE-SYNTHETASE FUNCTIONS IN ESCHERICHIA-COLI, BUT THE ENZYME IS NOT ADENYLYLATED [J].
FISHER, R ;
TULI, R ;
HASELKORN, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (06) :3393-3397
[5]   PURIFICATION AND CHARACTERIZATION OF GLUTAMINE-SYNTHETASE FROM THE UNICELLULAR CYANOBACTERIUM ANACYSTIS-NIDULANS [J].
FLORENCIO, FJ ;
RAMOS, JL .
BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 838 (01) :39-48
[6]   THE ROLE OF SPECIFIC CATIONS IN REGULATION OF CYANOBACTERIAL GLUTAMINE-SYNTHETASE [J].
IP, SM ;
ROWELL, P ;
STEWART, WDP .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 114 (01) :206-213
[7]  
LAURELL CB, 1981, METHOD ENZYMOL, V73, P339
[8]  
LOPEZRUIZ A, 1989, PLANT PHYSIOL BIOCH, V27, P461
[9]   METHODS FOR ENHANCEMENT OF IMAGE DETAIL AND ACCENTUATION OF STRUCTURE IN ELECTRON MICROSCOPY [J].
MARKHAM, R ;
FREY, S ;
HILLS, GJ .
VIROLOGY, 1963, 20 (01) :88-&
[10]   AMMONIA ASSIMILATING ENZYMES FROM CYANOBACTERIA - INSITU AND INVITRO ASSAY USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
MARQUES, S ;
FLORENCIO, FJ ;
CANDAU, P .
ANALYTICAL BIOCHEMISTRY, 1989, 180 (01) :152-157