SYNTHESIS OF 9-(BETA-D-ARABINOFURANOSYL)GUANINE USING WHOLE CELLS OF ESCHERICHIA-COLI

被引：28

作者：

ZINCHENKO, AI ^{[1
]}

BARAI, VN ^{[1
]}

BOKUT, SB ^{[1
]}

KVASYUK, EI ^{[1
]}

MIKHAILOPULO, IA ^{[1
]}

机构：

[1] ACAD SCI BESSR,INST BIOORGAN CHEM,MINSK,BELORUSSIA,USSR

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1990年 / 32卷 / 06期

关键词：

D O I：

10.1007/BF00164735

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Synthesis of 9-(β-d-arabinofuranosyl)guanine (ara-G) from 1-(β-d-arabinofuranosyl)cytosine (ara-C) and guanine, guanosine or 2′-deoxyguanosine (dG) by glutaraldehyde-treated Escherichia coli BM-11 cells is described. It is shown that the concentration of phosphate ions, molar ratio of substrates and pH of the reaction medium are factors affecting product yield. Under optimum conditions ara-G was produced in the reaction mixture in a yield of 63%-65% based on dG as the best source of guanine base. The yield of isolated ara-G was 48%-53%. © 1990 Springer-Verlag.

引用

页码：658 / 661

页数：4

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