HIGH-AFFINITY BINDING OF OESTRADIOL-17-BETA IN THE NUCLEI OF HUMAN ENDOMETRIAL CELLS

The development of a specific assay for high affinity nuclear receptors for use in human endometrium is described. A nuclear suspension and 700 g supernatant were prepared from 150 mg of fresh tissue and aliquots incubated at 4°C for 18 h in the presence of 0.1-1.0 nM [3H]-oestradiol-17β. Loosely bound [3H]-oestradiol-17β was removed from the nuclear preparation by washing in a millipore filter unit and from the 700 g supernatant by incubation with dextran-charcoal at 2°C for 15 min. Preliminary results obtained in normal human endometrium and in endometrial carcinoma are presented. © 1979.