ISOLATION AND CELL-WALL REGENERATION OF PROTOPLASTS FROM BOTRYTIS-CINEREA PERS

被引：3

作者：

BRAUN, PJ

HEISLER, A

机构：

[1] Department of Biological Research, Agricultural Division, Hoechst AG, Frankfurt

来源：

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT | 1990年 / 128卷 / 04期

关键词：

D O I：

10.1111/j.1439-0434.1990.tb04277.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Abstract A procedure for efficient isolation and cell wall regeneration of protoplasts from Botrytis cinerea is described. Protoplasts were obtained from mycelia using a lytic enzyme mixture containing β‐Glucuronidase, Cellulase R10 and Driselase with mannitol for osmotic support. The digestion of cell walls was checked by fluorescence microscopy. Protoplasts were purified from cell debris and lytic enzymes. Regeneration and reversion were performed by incubation on agar plates. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：293 / 298

页数：6

共 8 条

[1] CONDITIONS FOR EFFICIENT ISOLATION AND REGENERATION OF PROTOPLASTS FROM FULVIA-FULVA [J].

HARLING, R ;

KENYON, L ;

LEWIS, BG ;

OLIVER, RP ;

TURNER, JG ;

CODDINGTON, A .

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT, 1988, 122 (02) :143-146

[2]

KUNOH H, 1984, T MYCOL SOC JPN, V25, P413

[3]

KUNOH H, 1983, T MYCOL SOC JPN, V24, P341

[4]

KUNOH H, 1986, T MYCOL SOC JPN, V27, P349

[5]

Peberdy J.F., 2020, FUNGAL PROTOPLASTS, P31

[6] FUNGAL PROTOPLASTS - ISOLATION, REVERSION, AND FUSION [J].

PEBERDY, JF .

ANNUAL REVIEW OF MICROBIOLOGY, 1979, 33 :21-39

[7]

SHIRANE N, 1986, Annals of the Phytopathological Society of Japan, V52, P721, DOI 10.3186/jjphytopath.52.721

[8] STUDIES ON CUTIN-ESTERASE .2. CHARACTERISTICS OF CUTIN-ESTERASE FROM BOTRYTIS-CINEREA AND ITS ACTIVITY ON TOMATO-CUTIN [J].

SHISHIYA.J ;

ARAKI, F ;

AKAI, S .

PLANT AND CELL PHYSIOLOGY, 1970, 11 (06) :937-+

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