CLONING AND EXPRESSION OF A CDNA-ENCODING A NOVEL HUMAN NEUROTROPHIC FACTOR

被引：180

作者：

KAISHO, Y

YOSHIMURA, K

NAKAHAMA, K

机构：

[1] Biotechnology Research Laboratories, Takeda Chemical Industries Ltd, Yodogawa-ku, Osaka, 532

来源：

FEBS LETTERS | 1990年 / 266卷 / 1-2期

关键词：

cDNA cloning; Gene family; Glioma cell; Novel neurotrophic factor;

D O I：

10.1016/0014-5793(90)81536-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding a novel human neurotrophic factor (designated nerve growth factor-2; NGF-2) was cloned from a human glioma cDNA library using a synthetic DNA corresponding to human nerve growth factor (NGF). The cloned cDNA encodes a polypeptide composed of 257 amino acid residues including a prepro-sequence of 138 residues and a mature region of 119 residues. The amino acid sequence of human NGF-2 exhibits 58% similarity with that of human NGF. Conditioned medium of COS-7 cells transfected with an expression plasmid for human NGF-2 cDNA supported the survival of sensory neurons isolated from dorsal root ganglia of embryonic chicks. A 1.5 kb of NGF-2 mRNA can be detected from an early development stage in rat brain, by Northern blotting analysis. © 1990.

引用

页码：187 / 191

页数：5

共 19 条

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