CLINICAL AND EXPERIMENTAL STUDIES ON THE ROLE OF PLATELET-ACTIVATING-FACTOR (PAF) IN THE PATHOGENESIS OF SEPTIC DIC

被引：8

作者：

ONO, S

TAMAKUMA, S

MOCHIZUKI, H

KINOSHITA, M

OHKUSA, Y

AOSASA, S

ODA, Y

OHE, H

机构：

[1] The First Department of Surgery, National Defense Medical College, Saitama, 359, 3-2 Namiki, Tokorozawa

[2] the Department of Physical and Analytical Chemistry, Tsukuba Research Laboratories, Eizai Company, Limited, Tsukuba

来源：

SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY | 1993年 / 23卷 / 03期

关键词：

PLATELET ACTIVATING FACTOR (PAF); ENDOTOXIN (ET); SEPTIC DISSEMINATED INTRAVASCULAR COAGULATION (SEPTIC DIC) ANTI-PAF AGENT; PROTEASE INHIBITOR;

D O I：

10.1007/BF00309232

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Various toxic factors induced by endotoxin (Et) are thought to be deeply involved in the pathogenesis of severe infections. In this study, particular attention was paid to the role of the platelet-activating factor (PAF) in these conditions, and clinical and experimental studies were conducted on the relationship between PAF and the changes observed in the general parameters after surgical infections. In the clinical study, changes in the PAF concentration in the blood of seven patients with disseminated intravascular coagulation (DIC), five of whom were septic and two non-septic, were monitored by gas/mass spectrometry. The mean PAF level in the septic DIC group tended to be higher than that in the non-septic DIC group. Moreover, in the septic DIC group, the relationship between the increase in the PAF level and platelet count was analyzed with the lapse of time and we surmised a negative correlation between these parameters. Experimentally, we also investigated the role of PAF in Et shock and the effect of an anti-PAF agent and protease inhibitor. The Et-induced fall in blood pressure was similarly prevented by both the anti-PAF agent and protease inhibitor. However, the decrease in the platelet count was more significantly inhibited by the anti-PAF agent than by the protease inhibitor, whereas the parameters of the blood coagulation/fibrinolysis system were more affected by the protease inhibitor than by the anti-PAF agent.

引用

页码：228 / 233

页数：6

共 12 条

[1]

Shiba T., Ikeda M., Yoshida H., Hara A., Takagi S., Hori J., Horiyama H., Takeuchi S., Potential role of platelet-activating factor in endotoxic shock, Saishin Igaku, 45, pp. 552-557, (1990)

[2]

Hosford D., Paubert-Braquet M., Braquet P., Platelet-activating factor and cytokine interactions in shock, Resuscitation, 18, pp. 207-218, (1989)

[3]

Yamada K., Asano O., Yoshimura T., Katayama K., Highly sensitive gas chromatographic-mass spectrometric method for the determination of platelet-activating factor in human blood, J Chromatogr, 433, pp. 243-247, (1988)

[4]

Terashita Z., Imura Y., Nishikawa K., Inhibition by CV-3988 of the binding of [3H] platelet-activating factor (PAF) to the platelet, Biochem Pharmacol, 34, pp. 1491-1495, (1985)

[5]

Nishikawa K., Possible pathophysiological roles of platelet-activating factor (PAF) in severe infection, Ther Res, 5, pp. 967-974, (1989)

[6]

Karasawa K., Nojima S., Assay methods for measuring PAF, Saishin Igaku, 45, pp. 449-455, (1990)

[7]

Tamakuma S., Ono S., Recent advanced therapy of surgical DIC, Med Postgrad, 29, pp. 191-195, (1991)

[8]

Sakaguchi K., Masugi F., Inoue M., Ogihara T., Yamada K., Yamatsu I., Direct evidence of elevated levels of circulating platelet-activating factor in a patient with disseminated intravascular coagulation syndrome, J Lipid Mediators, 1, pp. 171-173, (1989)

[9]

Ono S., Tamakuma S., Endotoxin shock, Gastroenterology, 9, pp. 134-140, (1988)

[10]

Imura Y., Terashita Z., Nishikawa K., Possible role of platelet-activating factor (PAF) in disseminated intravascular coagulation (DIC), evidenced by use of a PAF antagonist, CV3988, Life Sci, 39, pp. 111-117, (1986)

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