REDUCED BINDING-ACTIVITY OF TRANSCRIPTION FACTOR NF-KAPPA-B ACCOUNTS FOR MHC CLASS-I REPRESSION IN ADENOVIRUS-TYPE-12 E1-TRANSFORMED CELLS

被引：31

作者：

MEIJER, I ^{[1
]}

BOOT, AJM ^{[1
]}

MAHABIR, G ^{[1
]}

ZANTEMA, A ^{[1
]}

VANDEREB, AJ ^{[1
]}

机构：

[1] LEIDEN UNIV,SYLVIUS LAB,MOLEC CARCINOGENESIS LAB,POB 9503,2300 RA LEIDEN,NETHERLANDS

来源：

CELLULAR IMMUNOLOGY | 1992年 / 145卷 / 01期

关键词：

D O I：

10.1016/0008-8749(92)90312-D

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The early region 1 (E1) of human adenovirus (Ad) type 12 represses the expression of major histocompatibility (MHC) Class I genes in transformed primary rodent cells. In this paper we show that both NF-κB and KBF1 (p50 dimer) binding activity to the H2TF1 element in the Class I promoter is reduced in Ad12-13S-E1A-transformed cells compared to Ad5E1- or Ad12-12S-E1A-transformed cells. Consistently, in Ad 12E1A-13S-transformed cells the H2TF1 element does not contribute to transcriptional activity in transient expression assays, whereas it does contribute in Ad12E1A-12S-transformed cells. Therefore, the most likely explanation is that reduced binding of NF-κB and KBF1 to the H2TF1 element accounts for the down-regulation of MHC Class I expression in Ad12E1- and Ad12E1A-13S-transformed cells. © 1992.

引用

页码：56 / 65

页数：10

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