TIME-COURSE OF FORMATION OF INOSITOL PHOSPHATES DURING ENZYMATIC-HYDROLYSIS OF PHYTIC ACID (MYOINOSITOL HEXAPHOSPHORIC ACID) BY PHYTASE DETERMINED BY CAPILLARY ISOTACHOPHORESIS

被引：10

作者：

BLATNY, P

KVASNICKA, F

KENNDLER, E

机构：

[1] UNIV VIENNA,INST ANALYT CHEM,A-1090 VIENNA,AUSTRIA

[2] INST CHEM TECHNOL,DEPT CARBOHYDREATE CHEM & TECHNOL,CR-16000 PRAGUE,CZECH REPUBLIC

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 679卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)80577-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary isotachophoresis with conductivity detection was applied to the investigation of the hydrolytic decomposition of phytic acid (myo-inositol hexaphosphoric acid) by phytase, and for the formation of the reaction products as a function of time. The quantitation of all analytes (besides phytic acid the mono- to pentaphosphorylated inositols and orthophosphate) can be carried out using two different buffer systems.

引用

页码：345 / 348

页数：4