THE NUCLEOTIDE-SEQUENCE OF A COMPLEMENTARY-DNA ENCODING FLAVERIA-BIDENTIS CARBONIC-ANHYDRASE

被引:10
作者
CAVALLARO, A [1 ]
LUDWIG, M [1 ]
BURNELL, J [1 ]
机构
[1] QUEENSLAND UNIV TECHNOL,CTR MOLEC BIOTECHNOL,BRISBANE 4001,QLD,AUSTRALIA
关键词
FLAVERIA BIDENTIS; CARBONIC ANHYDRASE; LEADER SEQUENCE; C-4; PLANT; TRANSIT PEPTIDE;
D O I
10.1016/0014-5793(94)00767-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated and characterised a cDNA clone encoding the cytosolic form of carbonic anhydrase in the leaves of Flaveria bidentis, a C-4 dicotyledonous plant. The deduced amino acid sequence is similar to the carbonic anhydrase found in the chloroplasts of C-3 dicotyledonous plants. Western blot analysis of crude leaf extracts of F. bidentis indicates that the leader sequence (equivalent to the transit peptide of the chloroplastic form of CA found in C-3 plants) is not removed following translation of mRNA.
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页码:216 / 218
页数:3
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