750 MHZ 1H-NMR SPECTROSCOPY OF HUMAN BLOOD-PLASMA

The application of high-resolution 750 MHz H-1-NMR spectroscopy to a biological fluid is demonstrated for the first time and its advantages over 600 MHz H-1 observation shown by reference to studies on human blood plasma. Improvements in signal dispersion were observed which facilitated improved signal assignments. Differences in lipid/lipoprotein signal line-widths between 600 and 750 MHz were noted indicating that ultrahigh field measurements may help to give insight into dynamic motional phenomena of lipids in whole plasma. The two-dimensional J-resolved (JRES) technique and spin-echo spectra measured at 750 MHz have enabled new signal assignments to be made in control plasma. The application of 750 MHz JRES to the clinical chemical problem of the detection of abnormal metabolites associated with chronic renal failure is also demonstrated.