CHROMATOGRAPHIC PURIFICATION OF TOXIN OF TYPE E CLOSTRIDIUM BOTULINUM

被引：6

作者：

EMODI, AS

LECHOWICH, RV

机构：

[1] Department of Food Science Michigan State University East Lansing

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 35卷 / 06期

关键词：

D O I：

10.1016/0006-291X(69)90692-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Clostridium botulinum Type E extracellular toxin was purified on DEAE cellulose columns following ammonium sulfate precipitation and dialysis. Two fractions, one toxic and one non-toxic, were obtained. Disc electrophoresis of the toxic fraction showed three bands, two of which were not toxic. Passage of the toxic fraction through a Sephadex G-200 column separated it into two fractions with equal toxicity. The estimated molecular weights of the two toxic fractions were about 9000 and 5000, respectively. © 1969.

引用

页码：788 / +

页数：1

共 8 条

[1] GEL-FILTRATION BEHAVIOUR OF PROTEINS RELATED TO THEIR MOLECULAR WEIGHTS OVER A WIDE RANGE [J].