PENETRATION OF DENTIN BY 3 ORAL BACTERIA INVITRO AND THEIR ASSOCIATED CYTOTOXICITY

被引：33

作者：

MERYON, SD

BROOK, AM

机构：

[1] Department of Oral Pathology, Dental School, University of Birmingham, Birmingham

来源：

INTERNATIONAL ENDODONTIC JOURNAL | 1990年 / 23卷 / 04期

关键词：

D O I：

10.1111/j.1365-2591.1990.tb00100.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Summary. Three common oral bacteria, namely Streptococcus sanguis. Actinomyces viscosus and Curynebacterium spp, were studied with regard to their ability to penetrate etched and unetched dentine and for their effect on underlying cell cultures. The test organisms were grown in cylinders above dentine slices 100 and 500 μm thick for 72 hours. The slices were in contact with tissue culture medium covering a layer of fibroblasts. Penetration of 100 μm slices was most rapid with 5. sanguis, followed by A. viscosus and Corynebacterium. The pattern was similar but slightly delayed when 500 μm slices were used, but in most cases penetration had occurred by 72 hours. The presence of a smear layer had no effect on the results obtained. Following penetration, cell destruction was most extensive with S. sanguis, the most cytotoxic organism, followed by Corynebacterium and A. viscosus. In the limited number of dishes where no penetration occurred there was little effect on cell numbers. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：196 / 202

页数：7

共 23 条

[1]

Akpata E.S., Blechman H., Bacterial invasion of pulpal dentin wall in vitro, Journal of Dental Research, 61, pp. 435-438, (1982)

[2]

Bergenholtz G., Effect of bacterial products on inflammatory reactions in the dental pulp, Scandinavian Journal of Dental Research, 85, pp. 122-129, (1977)

[3]

Bergenholtz G., Cox C.F., Loesche N.J., Syed S.A., Bacterial leakage around dental restorations: its effect on the dental pulp, Journal of Oral Pathology, 11, pp. 439-450, (1982)

[4]

Brannstrom M., Nordenvall K.J., Bacterial penetration, pulpal reaction and the inner surface of Concise Enamel Bond, composite fillings in etched and unetched cavities, Journal of Dental Research, 57, pp. 3-10, (1978)

[5]

Browne R.M., Tobias R.S., Crombie I.K., Plant C.G., Bacterial microleakage and pulpal inflammation in experimental cavities, International Endodontic Journal, 16, pp. 147-155, (1983)

[6]

Cox C.F., Keall C.L., Keall H.J., Ostro E., Bergenholtz G., Biocompatibility of surface‐sealed dental materials against exposed pulps, Journal of Prosthetic Dentistry, 57, pp. 1-8, (1987)

[7]

Duguid R., Lyons I.R., The effect of extracts from human dental plaque, oral streptococci and actinomyces bacteria on HeLa cell growth, [<sup>3</sup>H]‐thymidine uptake and <sup>51</sup>Cr release, Archives of Oral Biology, 27, pp. 65-68, (1982)

[8]

Eriksen H.M., Pulpal response of monkeys to a composite resin cement, Journal of Dental Research, 53, pp. 565-570, (1974)

[9]

Lundy T., Stanley H.R., Correlation of puipal histopathology and clinical symptoms in human teeth subjected to experimental irritation, Oral Surgery, Oral Medicine, Oral Pathology, 27, pp. 187-201, (1969)

[10]

Meryon S.D., Jakeman K.J., Browne R.M., Investigation of the cytotoxicity of bacterial species implicated in pulpal inflammation, International Endodontic Journal, 19, pp. 11-20, (1986)

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