TRANSCRIPTION OF THE BACILLUS-SUBTILIS-SPOIIA LOCUS

被引:69
作者
WU, JJ
PIGGOT, PJ
TATTI, KM
MORAN, CP
机构
[1] TEMPLE UNIV, HLTH SCI CTR,SCH MED,DEPT MICROBIOL & IMMUNOL, 3400 N BROAD ST, PHILADELPHIA, PA 19140 USA
[2] EMORY UNIV, SCH MED, DEPT MICROBIOL & IMMUNOL, ATLANTA, GA 30322 USA
关键词
RECOMBINANT DNA; SIGMA-H-RNA POLYMERASE; SPORULATION;
D O I
10.1016/0378-1119(91)90231-Y
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The sPoIIA operon encodes three genes, including the structural gene for a sporulation-induced sigma factor, sigma-F. We used deletion analysis of spoIIA-lacZ fusions to define the location of the spoIIA promoter. We found that sigma-H-RNA polymerase transcribes spoIIA accurately in vitro and propose that sigma-H directs transcription of spoIIA during sporulation.
引用
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页码:113 / 116
页数:4
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