INVITRO PHAGOCYTOSIS INHIBITION ASSAY FOR THE SCREENING OF POTENTIAL CANDIDATE ANTIGENS FOR SUBUNIT VACCINES AGAINST THE ASEXUAL BLOOD STAGE OF PLASMODIUM-FALCIPARUM

被引：40

作者：

GYSIN, J

GAVOILLE, S

MATTEI, D

SCHERF, A

BONNEFOY, S

MERCEREAUPUIJALON, O

FELDMANN, T

MULLERHILL, B

DASILVA, LP

机构：

[1] INST PASTEUR, IMMUNOPARASITOL LAB, CAYENNE, FRENCH GUIANA

[2] INST PASTEUR, EXPTL PARASITOL UNIT, F-75724 PARIS 15, FRANCE

[3] UNIV COLOGNE, DEPT GENET, W-5000 COLOGNE 41, GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1993年 / 159卷 / 1-2期

关键词：

PLASMODIUM-FALCIPARUM; PHAGOCYTOSIS; IMMUNOPROTECTION; OPSONIZING ANTIBODY;

D O I：

10.1016/0022-1759(93)90159-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously established a direct correlation between immune protection against the asexual blood stage Plasmodium falciparum infection and the presence of opsonizing antibodies promoting phagocytosis of parasitized red blood cells. In the present communication we describe an in vitro assay for measuring phagocytosis inhibition (PIA) specific for P. falciparum-infected erythrocytes. The phagocytosis inhibition assay is a simple procedure for screening potential candidates for sub-unit vaccines against P. falciparum based on the correlation between opsonizing antibodies and immunoprotection. The assay was used to analyse 18 recombinant molecules, corresponding to 11 distinct antigens of P. falciparum. Pre-incubation and selective antibody depletion experiments demonstrate the antigen-antibody specificity of the PIA. The presence of epitopes participating as targets of opsonic antibodies were demonstrated in six distinct polypeptide antigens.

引用

页码：209 / 219

页数：11

共 44 条

[1] KNOB-POSITIVE AND KNOB-NEGATIVE PLASMODIUM-FALCIPARUM DIFFER IN EXPRESSION OF A STRAIN-SPECIFIC MALARIAL ANTIGEN ON THE SURFACE OF INFECTED ERYTHROCYTES [J].