FAST-FREEZING DEVICES FOR CRYO-ELECTRON-MICROSCOPY

被引:12
作者
TRACHTENBERG, S
机构
[1] Department of Membrane, Ultrastructure Research The Hebrew University-Hadassah Medical School, Jerusalem, 91010
关键词
CRYOELECTRON MICROSCOPY; FAST-FREEZING; FREEZE-DRYING; FREEZE SUBSTITUTION; VITRIFICATION;
D O I
10.1016/0968-4328(93)90009-P
中图分类号
TH742 [显微镜];
学科分类号
摘要
The design, construction and performance of two simple fast-freezing devices are described. The devices are driven pneumatically at a controlled speed of up to 10 msec-1. They are suitable for either quench-freezing (in liquified gases) or for bounce-free impact-freezing on liquid nitrogen cooled metal blocks. Provisions are made to keep the metal block free of condensation and to allow multiple freezing.
引用
收藏
页码:1 / 12
页数:12
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